CRIMICtechnique (under construction)

Gene traps and protein traps are valuable genetic tools to study gene function. Most of these constructs are inserted in the genome as artificial exons. The artificial exon must be inserted in an intron between two coding exons to function as a gene or protein trap.

About 50% of the Drosophila genes do not have a suitable coding intron. We developed methods to replace the coding region of these genes with a Kozak-GAL4-3XP3GFP cassette creating a null allele, while expressing GAL4.