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Selected Publications

Gene tagging strategies

  • Kanca O, Zirin J, Hu Y, Tepe B, Dutta D, Lin WW, Ma L, Ge M, Zuo Z, Liu LP, Levis RW, Perrimon N, Bellen HJ (2022) An expanded toolkit for Drosophila gene tagging using synthesized homology donor constructs for CRISPR-mediated homologous recombination. eLife 11:e76077. PMID: 35723254.

  • Kanca O, Zirin J, Garcia-Marques J, Knight SM, Yang-Zhou D, Amador G, Chung H, Zuo Z, Ma L, He Y, Lin WW, Fang Y, Ge M, Yamamoto S, Schulze KL, Hu Y, Spradling AC, Mohr SE, Perrimon N, Bellen HJ (2019) An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms. eLife 8: e51539. PMID:31674908.

  • Li-Kroeger D*, Kanca O*, Lee PT, Cowan S, Lee MT, Jaiswal M, Salazar JL, He Y, Zuo Z, Bellen HJ (2018) An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. eLife 7: e38709. PMID:30091705.  (*equal contribution)

  • Kanca O, Bellen HJ, Schnorrer F (2017) Gene tagging strategies to assess protein expression, localization, and function in Drosophila. Genetics 207:389-412.

Use of Drosophila for human research 

  • Yamamoto S*, Kanca O*, Wangler MF , Bellen HJ (2023) Integrating non-mammaliam model organism in the diagnosis of rare genetic human disease. Nature Review Genetics :doi: 10.1038/s41576-023-00633-6.

  • Guichard A*, Lu S*, Kanca O*, Bressan D, Huang Y, Ma M, Sanz Juste S, Andrews JC, Jay K, Sneider M, Schwartz R, Huang MC, Bei D, Pan H, Ma L, Lin W-W, Auradkar A, Bhagwat P, Park S, Wan KH, Ohsako T, Takano-Shimizu T, Celniker SE, Wangler MF, Yamamoto S#, Bellen HJ#, Bier E# (2023) A comprehensive Drosophila resource to identify key functional interactions between SARS-CoV-2 factors and host proteins. Cell Reports 42(8):112842.

  • Kanca O, Andrews JC, Lee PT, Patel C, Braddock SR, Slavotinek AM, Cohen J, Gubbels CS, Aldinger KA, Williams J, Indaram M, Fatemi A, Yu TW, Agrawal PB, Vezina G, Wynn J, Hernan R, Mychaliska G, Undiagnosed Diseases Network, Chung WK, Markello TC, Dobyns WB, Adams DR, Gahl WA, Wangler MF, Yamamoto S, Bellen HJ, Malicdan MCV (2019) De novo variants in WDR37 are associated with epilepsy, colobomas, dysmorphism, developmental delay, intellectual disability, and cerebellar hypoplasia. Am J Hum Genet 105(2): 413-424. PMID:31491411.

  • Dutta D, Kanca O, Byeon SK, Marcogliese PC, Zuo Z, Shridharan RV, Park JH, Undiagnosed Diseases Network, Lin G, Ge M, Heimer G, Kohler JN, Wheeler MT, Kaipparettu BA, Pandey A, Bellen HJ (2023) A defect in mitochondrial fatty acid synthesis impairs iron metabolism and causes elevated ceramide levels. Nature Metabolism 5(9):1595-1614

Diverse genetic tools

  • Kanca O, Caussinus E, Denes AS, Percival-Smith A, Affolter M. 2013. Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development 141(2): 472-480. PMID: 24335257.

  • Caussinus E, Kanca O, Affolter M. 2012. Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Nature Structural & Molecular Biology 19(1): 117-121. PMID: 22157958.

  • Bieli D, Kanca O, Requena D, Hamaratoglu F, Gohl D, Schedl P, Affolter M, Slattery M, Müller M, Estella C. 2015. Establishment of a developmental compartment requires interactions between three synergistic cis-regulatory modules. PLoS Genetics 11(10): e1005376. PMID:26468882

  • Bieli D, Kanca O, Gohl D, Denes A, Schedl P, Affolter M, Müller M. 2015. The Drosophila melanogaster Mutants apblot and apXasta affect an essential apterous wing enhancer. G3 (Bethesda) 5(6): 1129-1143. PMID: 25840432.

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